微信小李
微信小章
产品简介
购买须知
| 产品名称： | JURKAT |
|---|---|
| 商品货号： | TS259326 |
| <strong>Cell line </strong>： | JURKAT |
| DSMZ no. ： | TS259326 |
| Species ： | originally confirmed as human with IEF of AST, LDH, NP and recently by STR fingerprinting |
| Cell type ： | T cell leukemia |
| Origin ： | established from the peripheral blood of a 14-year-old boy with acute lymphoblastic leukemia (ALL) at first relapse in 1976; often this cell line is called "JM" (JURKAT and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14944, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or in clumps in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at about 1 x 106 cells/ml; optimal split ratio of about 1:2 to 1:3 every 2-3 days; maintain at 0.5-1.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 25-35 hours |
| Harvest ： | maximal density at about 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 +, CD3 +, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, TCRalpha/beta +, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype with 7.8% polyploidy - 87(78-91)<4n>XX, -Y, -Y, -5, -16, -17, -22, add(2)(p21)/del(2)(p23)x2 - sideline with additional der(5)t(5;10)(q11;p15), del(9)(p11) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |