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| 产品名称： | SF-158 |
|---|---|
| 商品货号： | TS259329 |
| <strong>Cell line </strong>： | SF-158 |
| DSMZ no. ： | TS259329 |
| Species ： | originally primate and rodent species were excluded by IEF and species PCR, and recently Spodoptera frugiperda origin confirmed by COI DNA barcoding |
| Cell type ： | |
| Origin ： | cloned from the parent line, IPLB-SF-21-AE, derived from pupal ovarian tissue of the fall armyworm (species Spodoptera frugiperda, family Noctuidae, order Lepidoptera); cells are susceptible to infection with MNPV and other Baculoviruses; cells can be used with Baculovirus expression vectors |
| Reference(s) ： | 14532 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic round cells, mostly adherent, a few cells in suspension |
| Medium ： | 80-90% TC 100 medium + 10-20% h.i. FBS (or 80-90% Grace´s insect medium + 10-20% h.i. FBS supplemented with L-Glutamine, hydrolyzed lactalbumin and yeastolate) |
| Subculture ： | split culture 1:4 to 1:5 every 5-6 day (cells grow adherent, but trypsin is not necessary as cells can be detached by tapping the flask or with a rubber scraper); seed out about 1-2 x 106 cells/25 cm2 |
| Incubation ： | 25-29 °C without CO2 (keep lid of flask closed); temperature below 23 °C may lead to reduced growth and loss of adherence |
| Doubling time ： | about 70 hours (at room temperature) |
| Harvest ： | cell harvest of about 0.2 x 106 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses ： | PCR: SMRV - |