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| 产品名称： | RPMI-8402 |
|---|---|
| 商品货号： | TS259331 |
| <strong>Cell line </strong>： | RPMI-8402 |
| DSMZ no. ： | TS259331 |
| Species ： | originally confirmed as human with IEF of AST, MDH |
| Cell type ： | T cell acute lymphoblastic leukemia |
| Origin ： | established from the peripheral blood of a 16-year-old woman with acute lymphoblastic leukemia (ALL) in 1972. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14619, 15152, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | roundish cells growing singly or in small clumps in suspension; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at about 0.2 -0.3 x 106 cells/ml; optimal split ratio of 1:3 to 1:7 once a week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 48 hours |
| Harvest ： | saturation density at about 1-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contaminaton was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 +, CD3 -, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 +, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotetraploid karyotype with 2% polyploidy - 90(79-91)<4n>XXX, -X, +3, +3, -10, -13, -14, +15, -18, -20, +2mar, dup(4)(q13q23)x2, del(6)(q14q22)x2, t(11;14)(p15;q11)x2, add(15)(p13) - sideline with der(1)t(1;9)(p35/36;q11), add(13)(q34) - carries t(11;14) with LMO1-TRD (LMO1-TCRD) rearrangement and cryptic del(1)(p32) effecting STIL-TAL1 (SIL-TAL1) fusion, resembles published karyotypes |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |