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| 产品名称： | U-138-MG |
|---|---|
| 商品货号： | TS259332 |
| <strong>Cell line </strong>： | U-138-MG |
| DSMZ no. ： | TS259332 |
| Species ： | confirmed as human with IEF of AST, LDH, MDH, NP |
| Cell type ： | glioblastoma |
| Origin ： | established from the glioblastoma of a 47-year-old Caucasian man |
| Reference(s) ： | 14374, 14856 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblastoid fusiform cells growing as mono- or multilayers; image; image |
| Medium ： | 90% Hams F10 + 10% h.i. FBS |
| Subculture ： | seed out at about 1-2 x 106 cells/80cm2 flask; split confluent culture 1:5 to 1:10 every 4-8 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | cell harvest of ca. 4-6 x 106/80cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 20% polyploidy - 60(57-63)<3n>XYY, -3, -5, -6, -8, -9, -10, -11, -13, -14, -17, -19, +2mar, add(4)(p15), del(6)(q15), dup(7)(q35q21), add(11)(p15), add(12)(p11), add(14)(q32) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |