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| 产品名称： | RV-C2 |
|---|---|
| 商品货号： | TS259335 |
| <strong>Cell line </strong>： | RV-C2 |
| DSMZ no. ： | TS259335 |
| Species ： | confirmed as murine with IEF of AST, LDH, MDH and NP and recently as mus musculus by COI DNA barcoding |
| Cell type ： | hybridoma (anti-rat fiber/troponin) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG2b) reacting with fetal, neonatal and adult cardiac fibers in immunofluorescence and with rat cardiac fetal and adult troponin T in Western blotting; in immunofluorescence this antibody stains some rat fetal and neonatal skeletal muscle fibers which express fetal cardiac troponin T as also shown by the Western blotting of rat fetal and neonatal skeletal muscle; this cell line was produced by fusing NSO myeloma cells with spleen cells from a Balb/c mouse that had been immunized with a myofibrillar preparation from hypothyroid rabbit ventricle |
| Reference(s) ： | 14910, 14908 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round single cells in suspension |
| Medium ： | 80% Dulbeccos MEM (4.5 g/L glucose) + 20% horse serum + 2 mM L-glutamine |
| Subculture ： | optimal split ratio of 1:2 to 1:3 twice a week; seed out at about 0.5-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 32 hours |
| Harvest ： | maximal density at about 2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% horse serum, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | secretion of a IgG2b monoclonal antibody |
| Cytogenetics ： | murine hypertetraploid/pentaploid karyotype - 88-99<4n> - 2 centric fusion markers and 1-2 dmin present in most cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |