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| 产品名称： | P-388D1(IL-1) |
|---|---|
| 商品货号： | TS259337 |
| <strong>Cell line </strong>： | P-388D1(IL-1) |
| DSMZ no. ： | TS259337 |
| Species ： | originally confirmed as mouse with IEF of LDH, MDH, NP, by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | monocytes-macrophages |
| Origin ： | established from the ascites of a DBA/2 mouse with a lymphoid neoplasm induced by methylcholanthrene and subsequently enriched for adherent (monocyte-macrophage) cells by selection (yielding this IL1-producing subclone); reported to produce IL-1 after stimulation with LPS or PMA, to phagocytize latex particles and to synthesize lysozyme |
| Reference(s) ： | 14436, 14687, 15131 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent elliptical and round loosely adherent cells growing in monolayers; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% horse serum + 3.5 g/L glucose |
| Subculture ： | seed out at ca. 1-3 x 106 cells/175cm2 flask; split confluent culture 1:3 to 1:6 every 3-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-60 hours (according to density) |
| Harvest ： | cell harvest of ca. 25 x 106 cells/175cm2 flask |
| Storage ： | frozen with 70% medium, 15% horse serum, 5% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypertriploid karyotype with 8% polyploidy - 61(57-65)<3n> - 5-8 centric fusion markers |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |