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| 产品名称： | A4-1025 |
|---|---|
| 商品货号： | TS259339 |
| <strong>Cell line </strong>： | A4-1025 |
| DSMZ no. ： | TS259339 |
| Species ： | originally confirmed as mouse with IEF of AST, NP and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (anti-fiber/myosin) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG2a) detecting all fibers in skeletal muscle and myosin heavy chains on Western blots and detecting mammalian, chicken, zebrafish, axolotl, xenopus skeletal muscle myosin heavy chains; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin |
| Reference(s) ： | 14444 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round single cells in suspension, partly in clusters, lightly adherent |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids |
| Subculture ： | split saturated culture 1:5 to 1:10 every 2-3 days; seed out at about 1.0-1.5 x 105 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 40-50 hours |
| Harvest ： | cell harvest of about 0.5-1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | secretion of a IgG2a monoclonal antibody |
| Cytogenetics ： | murine hypertetraploid karyotype with 5% polyploidy - 84(79-84) - one large submetacentric and up to 5 centric fusion markers present |
| Viruses ： | ELISA: reverse transcriptase positive |