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| 产品名称： | SW-403 |
|---|---|
| 商品货号： | TS259341 |
| <strong>Cell line </strong>： | SW-403 |
| DSMZ no. ： | TS259341 |
| Species ： | confirmed as human with IEF of AST, LDH |
| Cell type ： | colon adenocarcinoma |
| Origin ： | established from the adenocarcinoma (grade III) of a 51-year-old Caucasian woman; cells were described in the literature to produce carcinoembryonic antigen (CEA) and to be tumorigenic in nude mice |
| Reference(s) ： | 14713 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent round cells, sometimes aggregated in complexes of 20-100 cells |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:3 to 1:4 every 3-4 days using trypsin/EDTA; seed out at about 2.5-3.0 x 106 cells/25 cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 40-50 hours |
| Harvest ： | cell harvest of about 40 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 12% polyploidy - 62(58-70)<3n>XX/XXX, +2, +2, -8, -9, -10, -10, -12, -13, -14, -18, -19, -22, +mar, der(1)t(1;1)(p21;q22), del(4)(q21), add(5)(p15), del(6)(q24-q25), i(8q), del(9)(p21), i(9q), add(13)(p13), add(14)(p13) - carries very large isochromosome-marker, does not correspond to published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |