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| 产品名称： | SK-MEL-1 |
|---|---|
| 商品货号： | TS259351 |
| <strong>Cell line </strong>： | SK-MEL-1 |
| DSMZ no. ： | TS259351 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | melanoma |
| Origin ： | established from the lymph node metastasis (thoracic duct) of a 29-year-old Caucasian man with primary melanoma in 1966; cells were described in the literature to produce pigment and to lead to pigment-producing tumors upon heterotransplantation into hamster |
| Reference(s) ： | 14810 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly round cells growing singly or in clumps (occasional giant, polynucleated cells) in suspension |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | maintain between 0.1-0.3 x 106 cells/ml; seed out at 0.2 x 106 cells/ml; due to rapid acidification of the medium, spent culture medium should be exchanged frequently; a cell density of 0.5 x 106 cells/ml should not be exceeded |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 100 hours |
| Harvest ： | ca. 0.5-1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 4% polyploidy - 78(72-79)<3n>XXYY, +X/Y, +2, +4, -5, +6, +7, +11, -12, -13, -13, +15, -17, +19, -21, +6mar - add(2)(p24), add(3)(q11), i(4q)x2, del(6)(q22), add(6)(q27), add(7)(q35)x2, del(9)(p23)x2, add(10)(p13), del(11)(p15), del(12)(q24), add(14)(p11), add(14)(q32), i(17q), add(17)(p12), add(19)(q13), del(?22)(q11) - carries pair of large submetacentric markers - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative: PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |