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| 产品名称： | HC-1 |
|---|---|
| 商品货号： | TS259352 |
| <strong>Cell line </strong>： | HC-1 |
| DSMZ no. ： | TS259352 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | hairy cell leukemia |
| Origin ： | spontaneously immortalized peripheral blood leukocytes from a 56-year-old white man with hairy cell leukemia (HCL) at diagnosis; cells were described in the literature to grow in soft agar, to be tumorigenic in irradiated nude mice and to be negative for the HCL-associated mutation BRAF V600E. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14931, 16917, 18187 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoblastoid cells growing as single cells or in clumps, in suspension; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:5 every 2-4 days; maintain at 0.3-0.6 x 106 cells/ml; seed out at ca. 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 45 hours |
| Harvest ： | saturation density of ca. 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-tetraploid karyotype with 3% polyploidy - 92(89-92)<4n>XXYY, add(2)(p13->23) - differs from published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |