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| 产品名称： | BA/F3 |
|---|---|
| 商品货号： | TS259353 |
| <strong>Cell line </strong>： | BA/F3 |
| DSMZ no. ： | TS259353 |
| Species ： | originally confirmed as mouse with IEF of AST, MDH, NP, PEP B and with species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | pro B cells |
| Origin ： | IL-3 dependent murine pro B cell line; the precise origin of the cell line is unclear; however, performing May-Gruenwald-Giemsa staining provides evidence that the cells are indeed from a lymphatic back ground. Cells were apparently not derived from BALB/c, but from C3H mouse. Direct publication is not available, but for related publications see references. |
| Reference(s) ： | 14829, 14830 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly single, round (some polymorph) cells in suspension (or occasionally in clumps); image; image; image |
| Medium ： | 90-95% RPMI 1640 + 5-10% h.i. FBS + 10 ng/ml mouse IL-3 or 10% vol conditioned medium of WEHI-3B cell line (DSM ACC 26) |
| Subculture ： | seed out at about 1-3 x 105 cells/ml; split saturated culture 1:10 every 3 days; at high cell density (>2 x 106 cells/ml), a cytokine-independent subclone may grow out relatively quickly. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 20 hours |
| Harvest ： | maximal density of about 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD11b (+), F4/80 (+); image |
| Cytogenetics ： | murine near diploid karyotype with 33% polyploidy - 40(36-42)<2n> |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |