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| 产品名称： | BM-1604 |
|---|---|
| 商品货号： | TS259356 |
| <strong>Cell line </strong>： | BM-1604 |
| DSMZ no. ： | TS259356 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | prostate carcinoma (derivative of DU-145) |
| Origin ： | cytogenetic analysis and DNA fingerprinting at the DSMZ documented that this cell line is identical with the human prostate carcinoma-derived cell line DU-145 (DSM ACC 261), but shows some phenotypical differences; DU-145 was established from the tumor tissue removed from the metastatic central nervous system lesion of a 69-year-old man with prostate carcinoma in 1975 |
| Reference(s) ： | 15040 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing as monolayers; image; image |
| Medium ： | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture ： | split confluent culture using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/175 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | doubling time highly variable, about 60 hours |
| Harvest ： | cell harvest of about 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as DU-145 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypotriploid karyotype with 22% polyploidy - 59(55-63)<3n>X, -X/Y, -Y, -2, -3, -4, -7, -8, -14, -16, -19, -20, -21, -22, +5mar (inc bisat x2-3), del(1)(p31), del(2)(p12), i(5p), del(6)(q22)/add(6)(q?12), der(6)t(4;6)(q22;p22.3), del(9)(p21), add(10)(q24-q25), add(11)(p11), t(11;?22)(q24;q13)/del(11)(q23), der(12)t(5;12)(q13;p12.3), add(13)(q33)x2, add(13)(q33), add(15)(p11), der(18)t(14;18) (q11;q12.3)x1-2 - sideline with del(2)(p23), i(8q) - closely related to DU-145 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |