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| 产品名称： | DV-90 |
|---|---|
| 商品货号： | TS259358 |
| <strong>Cell line </strong>： | DV-90 |
| DSMZ no. ： | TS259358 |
| Species ： | originally confirmed as human with IEF of AST, LDH, MDH, PEP-B and recently by STR fingerprinting |
| Cell type ： | lung adenocarcinoma |
| Origin ： | established from the pleural effusion of a 50-year-old man with lung adenocarcinoma (stage IV) in 1990; cells were described in the literature to be tumorigenic in nude mice |
| Reference(s) ： | 14779 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing in monolayers; image; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine + 1x non-essential amino acids |
| Subculture ： | seed out ca. 2 x 106 cells/25 cm2; split confluent culture 1:2 to 1:3 every 3-4 days using trypsin/EDTA; after seeding, cells may need 2-3 days to adhere |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 95 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 17% polyploidy - 46(41-47)<2n>X, -Y, +8, add(6)(q27) - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |