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| 产品名称： | EFM-192B |
|---|---|
| 商品货号： | TS259363 |
| <strong>Cell line </strong>： | EFM-192B |
| DSMZ no. ： | TS259363 |
| Species ： | confirmed as human with IEF of LDH, MDH, NP |
| Cell type ： | breast carcinoma |
| Origin ： | established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma; cells were described in the literature to overexpress the erB-2 gene; sister cell lines EFM-192A and -192C show differences in molecular aspects |
| Reference(s) ： | 15156 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid adherent cells growing as monolayers |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | subcultivation before reaching confluence; split 1:2 to 1:3 once a week using trypsin/EDTA; seed out at ca. 0.5-1.0 x 105 cells/cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 4-5 days |
| Harvest ： | cell harvest of ca. 2-4 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as EFM-192A/EFM-192C using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human heteroploid hypotriploid karyotype with 13% polyploidy - 62(58-62)<3n>XXX/XX, -X, +2, +4, +6, -7, -8, -8, -10, -11, -12, +14, +16, -17, -17, -17, -18, -18, -19, -20, -21, -22, +8mar, add(1)(p22-31), add(2)(q?35), del(3)(p22), add(5)(p15), del(6)(q25), add(10)(p11), add(13)(q32), add(14)(p12), add(15)(p12), der(15)t(10;15)(q11;p12), der(20)t(1;20)(q24;q12/13), der(21)t(7;21)(q11;p12)x2 - most cells carry a small ring and 1-2 large submetacentric 15p+ markers - closely related to EFM-192A - apparent ch 17 nullisomy |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |