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| 产品名称： | 293 |
|---|---|
| 商品货号： | TS259364 |
| <strong>Cell line </strong>： | 293 |
| DSMZ no. ： | TS259364 |
| Species ： | confirmed originally as human with IEF of AST, LDH |
| Cell type ： | embryonal kidney |
| Origin ： | established from a human primary embryonal kidney transformed by adenovirus type 5 (Ad 5); classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS) but not as GMO; cell line also known as HEK-293 (human embryonic kidney-293) |
| Reference(s) ： | 14573 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line was established by transfection of the cells with sheared DNA of adenovirus type 5. The cells contain 4 to 5 copies of the left end of the virus (12% of the viral genome) including E1a and E1b genes and one copy of the right end of the virus (10% of the genome) including the E4 gene. No active viruses are produced. No GMO. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastoid cells growing as monolayer; image; image |
| Medium ： | 90-95% Dulbeccos MEM (with 25mM HEPES) + 5-10% h.i. FBS |
| Subculture ： | seed out at about 2-3 x 106 cells/25 cm2; split confluent culture 1:5 to 1:6 every 2-3 days (without using trypsin/EDTA, cells can be detached by tapping the flask) |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 24-30 hours |
| Harvest ： | cell harvest of about 20 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-triploid karyotype with 6% polyploidy - 69(64-69)XX?X, +1, +6, -10, -13, -16, -18, -20, +21, +22, +mar, add(?X)(p22), inv(1)(p35q43), del(1)(p35), i(5p), del(6)(q11), add(8)(q24), add(13)(p13), add(17)(qter), der(?)t(5;?)(q13;?) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |