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| 产品名称： | CHP-126 |
|---|---|
| 商品货号： | TS259365 |
| <strong>Cell line </strong>： | CHP-126 |
| DSMZ no. ： | TS259365 |
| Species ： | confirmed as human with IEF of LDH, MDH, NP |
| Cell type ： | neuroblastoma |
| Origin ： | established from a large retroperitoneal mass excised from a 14-month-old girl with neuroblastoma (stage III) in 1973; NMYC and DDX1 have been reported to be amplified |
| Reference(s) ： | 14936 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | spheroidal cells growing as clumps in suspension; grows as semi-adherent monolayer on surfaces coated with poly-L-lysine; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | maintain at 0.5-1.5 x 106 cells/ml; optimal split ratio 1:2 to 1:3 every 3-5 days; resuspend clumps by gentle pipetting on each occasion; Viability may be low and growth may be slow after recovery from freezing (doubling time of ca. 150-200 h); growth may be encouraged by plating out on flasks coated with poly-L-lysine |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 120 -140 hours |
| Harvest ： | saturation density of ca. 1.5 - 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype - 46(44-46)<2n>X, -X, +mar, del(1)(p36), t(2;14)(q37;q21-23), dup(7)(p15p22), der(11)t(1;11)(q22;q24), del(12)(q24.32), add(13)(p13), add(14)(p12) - ca. 20-50 dmin - del(1p32-36) and dmin (MYCN amp) are typical of late-stage neuroblastoma |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |