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| 产品名称： | EFM-192C |
|---|---|
| 商品货号： | TS259369 |
| <strong>Cell line </strong>： | EFM-192C |
| DSMZ no. ： | TS259369 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | breast carcinoma |
| Origin ： | established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma; sister cell lines EFM-192A and -192B show differences in molecular aspects; erbB-2 gene is amplified and overexpressed |
| Reference(s) ： | 15156 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid adherent cells growing as monolayers |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | optimal split ratio of 1:2 to 1:3 once a week using trypsin/EDTA; seed out at ca. 5-8 x 104 cells/cm2 or 2 x 106 cells/25 cm2 flask |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 4 days |
| Harvest ： | cell harvest of ca. 2-4 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as EFM-192A/EFM-192B using multiplex PCR of minisatellite markers |
| Cytogenetics ： | highly rearranged human hypohexaploid karyotype with 10% polyploidy - 122-138<6n>XXXXX/XXXXXX - related to EFM-192A and EFM-192 B |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |