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| 产品名称： | ECV-304 |
|---|---|
| 商品货号： | TS259371 |
| <strong>Cell line </strong>： | ECV-304 |
| DSMZ no. ： | TS259371 |
| Species ： | confirmed as human with IEF of LDH, MDH, NP |
| Cell type ： | urinary bladder carcinoma (derivative of T-24) |
| Origin ： | described as being "spontaneously immortalized from the umbilical cord vein of a new-born Japanese boy in 1984"; cells were described in the literature to have a high proliferative potential in the absence of any growth factors; however, DNA fingerprinting at DSMZ showed clearly that this cell line is in reality a derivative of human urinary bladder carcinoma cell line T-24 |
| Reference(s) ： | 14465, 14723, 14999, 15000, 15001, 15190 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent growth as cobblestone monolayers |
| Medium ： | 80-90% Medium 199 (with HEPES buffer + Earles salts) + 10-20% h.i. FBS |
| Subculture ： | optimal split ratio of 1:10 to 1:20 once or twice a week using trypsin/EDTA; seed out ca. 1-2 x 104 cells/cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 2 days |
| Harvest ： | cell harvest of max. 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as T-24 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypertriploid karyotype with 6.7% polyploidy - 78(75-81)<3n>XX, -X, +1, -4, +5, +5, +5, +7, -8, -9, -10, +14, -15, -15, +17, +18, +20, +20, +20, +21, +22, +4-6mar - add(1)(p32)x1-2, i(1p), der(2q;5q), der(3)t(3;11)(p25;q13), i(5p)x2, inv(7)(p14q31), add(7)(q22), del(9)(p22), add(11)(p15), der(14)t(7;14)(q21;q24), add(18)(p11)x2, add(?19) (q11) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |