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| 产品名称： | RBL-2H3 |
|---|---|
| 商品货号： | TS259372 |
| <strong>Cell line </strong>： | RBL-2H3 |
| DSMZ no. ： | TS259372 |
| Species ： | confirmed as rat with IEF of AST, LDH, NP and by species PCR |
| Cell type ： | basophilic leukemia |
| Origin ： | the rat basophlic leukemia (RBL) line was originally obtained by Eccleston et al. and was maintained by serial passage in neonatal Wistar rats; four cell lines (named RBL I-IV) were started from this tumor between 1973 and 1975; the subline RBL-IV HR+ was derived from RBL-IV; further subcloning established the cell line RBL-2H3; cells are described in the literature to release histamine as an IgE-mediated reaction |
| Reference(s) ： | 14372, 14696, 14500, 14613 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, fibroblast-like cells growing mainly as monolayer and in a small extend as suspension culture |
| Medium ： | 70% MEM (with Earles salts) + 20% RPMI 1640 + 10% h.i. FBS; a higher proportion of RPMI 1640 may increase proliferation rate, but decreases histamine production |
| Subculture ： | split culture before confluency 1:5 every 3 days using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C (some researchers favour cultivation without CO2) |
| Doubling time ： | ca. 40--60 hours |
| Harvest ： | cell harvest of ca. 15-20 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | histamine release confirmed by depositor |
| Cytogenetics ： | rat hypertriploid karyotype - 68(62-72)<3n> - carries large metacentric marker |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |