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| 产品名称： | SW-480 |
|---|---|
| 商品货号： | TS259375 |
| <strong>Cell line </strong>： | SW-480 |
| DSMZ no. ： | TS259375 |
| Species ： | confirmed as human with IEF of AST, LDH, MDH |
| Cell type ： | colon adenocarcinoma |
| Origin ： | established from the tumor of a 50-year-old Caucasian man with colon adenocarcinoma (grade 4, Duke class B) |
| Reference(s) ： | 14713 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | endothelial-like adherent cells, mostly bipolar with microvilli growing in monolayers; image; image |
| Medium ： | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture ： | seed out at about 1.0 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 once a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | 25-30 hours |
| Harvest ： | cell harvest of about 10-15 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 12% polyploidy - 58(51-58)<3n>XX, -Y, -1, -3, -4, -5, -6, +7, -8, -10, -12, -15, -18, -19, +20, -22, der(1)t(1;9)(q22;q12), t(2;12)(q35;q13.1), del(5)(q13.2q32), add(7)(q35), dup(7)(q11.22q21.3), der(8)t(1;8)(p11;p11)t(1;9)(q22;q12), add(10)(p14), inv(14)(q13q32), del(18)(q12.2), der(19)t(6;19)(p11;q13), der(20)t(14;20)(q13;p13)x2 - identified by large submetacentric der(2)t(2;12) marker |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |