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| 产品名称： | F1-652 |
|---|---|
| 商品货号： | TS259377 |
| <strong>Cell line </strong>： | F1-652 |
| DSMZ no. ： | TS259377 |
| Species ： | originally confirmed as murine with IEF of AST, NP, LDH and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (anti-fiber/myosin) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG1) reacting with embryonic fibers in rodent and human muscle and with embryonic myosin heavy chain on Western blots; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin |
| Reference(s) ： | 14432 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round large cells, lightly adherent (can be detached without trypsin) |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids |
| Subculture ： | split saturated culture 1:3 to 1:5 once or twice a week; cultivate optimally between 0.1-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 30-36 hours |
| Harvest ： | maximal density of about 2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | secretion of a IgG1 monoclonal antibody |
| Cytogenetics ： | murine hypertetraploid karyotype - 87(72-90)<4n> - carries 2-3 centric fusion markers and 1-2 dm |
| Viruses ： | ELISA: reverse transcriptase positive |