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| 产品名称： | P-19 |
|---|---|
| 商品货号： | TS259380 |
| <strong>Cell line </strong>： | P-19 |
| DSMZ no. ： | TS259380 |
| Species ： | originally confirmed as murine with IEF of AST, LDH, MDH, NP and by species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | embryonal carcinoma |
| Origin ： | established from the teratocarcinoma induced in a C3H/He strain mouse |
| Reference(s) ： | 14753, 14905 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing as adherent monolayer |
| Medium ： | 80-90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10-20% h.i. FBS |
| Subculture ： | split confluent culture 1:10 to 1:20 once or twice a week using trypsin/EDTA; seed out at ca. 0.3-0.4 x 105 cells/cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 2-3 days |
| Harvest ： | cell harvest of max. 0.5 x 106 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine near-diploid karyotype with 16% polyploidy - 40(31-44)<2n> - one centric fusion marker - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |