微信小李
微信小章
产品简介
购买须知
| 产品名称： | HT-1080 |
|---|---|
| 商品货号： | TS259386 |
| <strong>Cell line </strong>： | HT-1080 |
| DSMZ no. ： | TS259386 |
| Species ： | originally confirmed as human with IEF of AST, LDH, NP and later by STR fingerprinting |
| Cell type ： | fibrosarcoma |
| Origin ： | established from the biopsy from a fibrosarcoma of a 35-year-old Caucasian man in 1972; patient did not receive any chemo- or radiotherapy |
| Reference(s) ： | 14881 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells growing as monolayers; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture up to 1:10 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 25 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | to inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 24% polyploidy - 46<2n>XY, der(5)t(5;5)(p15;q32), der(11)t(10;11)(q24;q11) - sideline with -13, del(1)(p32), del(1)(q24) - 5p15 is a putative recurrent breakpoint in fibrosarcoma |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II, MLV -, SMRV - |