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| 产品名称： | HPD-1NR |
|---|---|
| 商品货号： | TS259387 |
| <strong>Cell line </strong>： | HPD-1NR |
| DSMZ no. ： | TS259387 |
| Species ： | originally confirmed as Syrian (Golden) Hamster by species PCR and recently as Mesocricetus auratus origin by COI DNA barcoding |
| Cell type ： | pancreas ductal adenocarcinoma |
| Origin ： | established from the pancreatic ductal adenocarcinoma of a female Syrian (Golden) hamster (Mesocricetus auratus) induced by treatment with BHP (chemical carcinogen); sister cell line of HPD-2NR (DSM ACC 293); described in the literature to be homotransplantable (i.e. in other syngeneic hamsters) and to carry a mutation in exon 1 of the c-Ki-ras oncogene |
| Reference(s) ： | 14784 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial cells growing mostly in monolayers (about 2-5% very large cells), but also with formation of a three-dimensional grid-like structure with some round, only loosly bound cells |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | split confluent culture before reaching confluence 1:2 to 1:4 every 3-6 days using trypsin/EDTA; seed out at about 2 x 106 cells/80 cm2; cells have a high metabolic activity acidifying the culture medium; exchange spent medium every 2 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-70 hours |
| Harvest ： | cell harvest of about 6-7 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Fingerprint ： | identical DNA profile with HPD-1NR multilocus probe (gtg)5 |
| Cytogenetics ： | near triploid highly rearranged karyotype with 36% polyploidy - 60-66<3n>XX, -X - hamster markers present - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |