微信小李
微信小章
产品简介
购买须知
| 产品名称： | N2-261 |
|---|---|
| 商品货号： | TS259389 |
| <strong>Cell line </strong>： | N2-261 |
| DSMZ no. ： | TS259389 |
| Species ： | originally confirmed as mouse with IEF of MDH, NP and recently as mus musculus by COI DNA barcoding |
| Cell type ： | hybridoma (anti-fiber/myosin) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG1) reacting with neonatal and adult slow and IIA fibers in rat and human and with slow and IIA fast myosin heavy chains in Western blots; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin |
| Reference(s) ： | 14620 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells, lightly adherent |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids |
| Subculture ： | split saturated culture 1:5 to 1:10 every 2-3 days; seed out at about 0.2 x 106 cells/ml |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 20 hours |
| Harvest ： | maximal density of about 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | secretion of a IgG1 monoclonal antibody |
| Cytogenetics ： | murine hypertetraploid karyotype with 8.3% polyploidy - 88(85-92)<4n> - carries 1-3 centric fusion markers, 1-3 dm |
| Viruses ： | ELISA: reverse transcriptase positive |