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| 产品名称： | RAJI |
|---|---|
| 商品货号： | TS259397 |
| <strong>Cell line </strong>： | RAJI |
| DSMZ no. ： | TS259397 |
| Species ： | originally confirmed as human with IEF of LDH, NP, PEP B |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established from the left maxilla of a 12-year-old African boy with Burkitt lymphoma in 1963; first continuous human hematopoietic cell line; classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS); cells are described in the literature to carry t(8;14) leading to MYC-IGH fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14862, 15231, 18187 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round single cells in suspension (partly in clusters or clumps); image |
| Medium ： | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; split saturated culture at 1:4 to 1:8 twice a week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24-36 hours |
| Harvest ： | maximum density at ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylamda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype with 12% polyploidy - 89(80-91)<4n>XXYY, -4, -9, -12, -16, -18, -21, +3mar, add(4)(q35), add(6)(q27)x2, t(8;14)(q24;q32), der(8)t(8;14) (q24;q32)add(8)(?p12), del(9)(p23), der(14)t(8;14)(q24;q32) - carries t(8;14) associated with Burkitt lymphoma - closely resembles depositors karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |