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| 产品名称： | SW-948 |
|---|---|
| 商品货号： | TS259398 |
| <strong>Cell line </strong>： | SW-948 |
| DSMZ no. ： | TS259398 |
| Species ： | confirmed originally as human with IEF of AST, LDH, MDH and later by STR fingerprinting |
| Cell type ： | colon adenocarcinoma |
| Origin ： | established from the colon tumor of a 81-year-old Caucasian woman with colon adenocarcinoma (Duke C) |
| Reference(s) ： | 14713 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells grow adherent in monolayers in tight clusters or islands of epithelium-like cells; image; image; image |
| Medium ： | 90% Leibovitzs L-15 + 10% h.i. FBS |
| Subculture ： | split confluent culture optimally at 1:2 to 1:7 once or twice a week using trypsin/EDTA; seed out at ca. 6 x 104 cells/cm2 or 2 x 106 cells/25 cm2 flask |
| Incubation ： | at 37 °C without CO2 |
| Doubling time ： | 2-4 days |
| Harvest ： | cell harvest of 2.0-2.5 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 12% polyploidy - 68(65-70)<3n>XXX, +7, -8, -10, +13, +13, -16, +2mar, del(X)(q22.2), del(1)(p12), i(9q), add(17)(p12), add(19)(q13) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |