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| 产品名称： | SNB-19 |
|---|---|
| 商品货号： | TS259402 |
| <strong>Cell line </strong>： | SNB-19 |
| DSMZ no. ： | TS259402 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | astrocytoma (derivative of U-251 MG) |
| Origin ： | established from the surgical resection of a left parieto-occipital glioblastoma from a 47-year-old man in 1980; cells were described in the literature to secrete plasminogen activator, to be clonogenic in soft agar and to be tumorigenic in nude mice; DNA fingerprinting showed unequivocally that SNB-19 is a subclone of the astrocytoma cell line U-251 MG) |
| Reference(s) ： | 14581 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastic cells growing as monolayer with contact inhibition, occasional giant cells; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:10 every 3-5 days using trypsin/EDTA; seed out at ca. 2-4 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed an identical STR DNA profile as U-251 MG |
| Cytogenetics ： | human hypotriploid karyotype with 15% polyploidy - 63(58-63)<3n>XXY, +1, +7, -8, -10, -12, -13, -14, -15, -16, -18, -21, -22, +2mar, der(1)del(1)(q23)ins(1;4)(p32;q?23q27), del(1)(q13), del(4)(q23q27), del(4)(q28q35), add(8)(q24), add(11)(p15), der(19)add(19)(p13)add(19)(q13) - large submetacentric, der(19) and der(1) markers - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |