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| 产品名称： | SISO |
|---|---|
| 商品货号： | TS259404 |
| <strong>Cell line </strong>： | SISO |
| DSMZ no. ： | TS259404 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | cervix adenocarcinoma |
| Origin ： | established from the surgically removed, chemotherapy-resistant cervix adenocarcinoma of a 67-year-old Japanese woman (clinical stage FIGO Ib) |
| Reference(s) ： | 15160 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial growth in compact colonies; cells tend to pile up after confluency |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:5 to 1:10 every 5-7 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/175 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | cell harvest of ca. 40 x 106 cells/175 cm2 (ca. 2.3 x 105 cells/cm2) |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 12% polyploidy - 61<3n>XXX, -1, -2, -3, -4, -6, -8, -10, -13, -14, -16, -17, -19, -22, +4mar, add(X)(q28), add(5)(p15), add(6)(p23), del(9)(q34), add(11)(q24), del(12)(q15), der(12)t(12;17)(p12;q11), add(13)(p12), add(18)(q23)x1-2 - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |