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| 产品名称： | BETA-TC-3 |
|---|---|
| 商品货号： | TS259405 |
| <strong>Cell line </strong>： | BETA-TC-3 |
| DSMZ no. ： | TS259405 |
| Species ： | originally confirmed as mouse with IEF of AST, NP and by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | pancreas insulinoma (beta-tumor) |
| Origin ： | established from pancreatic insulinoma excised from a transgenic mouse carrying hybrid insulin-SV40 large T genes in 1988; described as producing pro-insulin I and II processed into mature insulin (inducible 30-fold by glucose); however, the DSMZ did not verify the production of insulin |
| Reference(s) ： | 14957, 14501 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing as multilayers; image |
| Medium ： | 82.5% Dulbeccos MEM (4.5 g/L glucose) + 15% horse serum + 2.5% h.i. FBS |
| Subculture ： | split confluent culture 1:5 to 1:10 every 2-5 days by mechanical disruption (trypsin/EDTA can be used for a very short time); seed out at ca. 1-4 x 106 cells/175 cm2; the DSMZ cannot guarantee the production of insulin. |
| Incubation ： | at 37 °C with 7.5-10% CO2 |
| Doubling time ： | ca. 34 hours |
| Harvest ： | cell harvest of ca. 2-3 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypotetraploid karyotype - 74(70-78)<4n> - 2-4 centric fusion markers and 1-3 dmin present |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |