微信小李
微信小章
产品简介
购买须知
| 产品名称： | SR-4987 |
|---|---|
| 商品货号： | TS259407 |
| <strong>Cell line </strong>： | SR-4987 |
| DSMZ no. ： | TS259407 |
| Species ： | originally confirmed as murine with IEF of AST, MDH and NP, by species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | stromal cells |
| Origin ： | established from the bone marrow of a female BDF/1 mouse in 1987; described in the literature to produce M-CSF, but not G-CSF, GM-CSF, or IL-3 |
| Reference(s) ： | 14847 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblast-like cells growing adherently in monolayers |
| Medium ： | 90% McCoys 5A + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:2 to 1:5 twice a week using trypsin/EDTA; seed out ca. 1.0-1.5 x 106 cells/25 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24-36 hours |
| Harvest ： | cell harvest of ca. 3.0-3.5 x 105/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypotetraploid karyotype - 70(59-70)<4n> - 1-3 centric fusion, and a small ring, markers present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |