微信小李
微信小章
产品简介
购买须知
| 产品名称： | JTC-27 |
|---|---|
| 商品货号： | TS259408 |
| <strong>Cell line </strong>： | JTC-27 |
| DSMZ no. ： | TS259408 |
| Species ： | confirmed as rat by IEF and species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | hepatoma |
| Origin ： | established from the transplantable rat ascites hepatoma AH-601 induced by feeding with aminoazotoluene; the hepatoma was passaged by intraperitoneal transplantation though JAR-2 rats |
| Reference(s) ： | 14621 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells growing in monolayers |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | split confluent culture 1:6 to 1:8 twice a week using trypsin/EDTA; seed out at ca. 2 x 104 cells/cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 37 hours |
| Harvest ： | cell harvest of maximally 2.5-3 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI,microbiological culture, RNA hybridization assays |
| Cytogenetics ： | rat hyperdiploid karyotype with 15% polyploidy - 47(45-49)<2n> |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |