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| 产品名称： | JTC-15 |
|---|---|
| 商品货号： | TS259410 |
| <strong>Cell line </strong>： | JTC-15 |
| DSMZ no. ： | TS259410 |
| Species ： | confirmed as rat with IEF of MDH, NP |
| Cell type ： | hepatoma |
| Origin ： | established in 1963 from the ascites of a rat with hepatoma induced with 4-dimethylaminoazobenzene |
| Reference(s) ： | 15002 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial cells growing in monolayers |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:5 to 1:8 every 4-5 days using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/175 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 30 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics ： | rat hyperdiploid/hypotriploid karyotype with 33% polyploidy - 51(51-58)<2n> - secondary constriction marker present - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |