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| 产品名称： | VA-ES-BJ |
|---|---|
| 商品货号： | TS259414 |
| <strong>Cell line </strong>： | VA-ES-BJ |
| DSMZ no. ： | TS259414 |
| Species ： | confirmed originally as human with IEF of AST, MDH, later by STR typing |
| Cell type ： | epithelioid sarcoma |
| Origin ： | established from the bone marrow metastasis of an epithelioid sarcoma (epidural tumor) of a 41-year-old man following unsuccessful chemotherapy in 1991; cells were described in the literature to grow rapidly after heterotransplantation into nude mice, to secrete functional GM-CSF and to be positive for AE-1, AE-3, vimentin and epithelial membrane antigen |
| Reference(s) ： | 15138 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing in monolayers - epitheloid, partly fibroblastoid large cells |
| Medium ： | 80% Dulbeccos MEM + 10-20% h.i. FBS + 1% non-essential amino acids + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:2 to 1:5 once or twice a week |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 60-90 hours |
| Harvest ： | cell harvest of max. 1.5 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 13% polyploidy - 68(65-70)<3n>XX, -Y, -3, -4, +6, +7, +7, -11, -12, +13, -14, -15, +17, +20, -22, add(1)(p32-34), add(1)(q43)x2, add(9)(q34)x3, del(22)(q13.2) - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |