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| 产品名称： | M1 |
|---|---|
| 商品货号： | TS259415 |
| <strong>Cell line </strong>： | M1 |
| DSMZ no. ： | TS259415 |
| Species ： | originally confirmed as murine with IEF of MDH, NP and recently as mus musculus by COI DNA barcoding |
| Cell type ： | myeloid leukemia |
| Origin ： | bone marrow from SL mouse with spontaneous myeloid leukemia was injected into isogenic mice and the cell line was established from the ensuing hyperplastic lymph nodes 14 days later by recloning on soft agar; homotransplantable and forms colonies on soft agar (granulocytic-macrophage differentation inducible by IL-3, LPS or dexamethasone) |
| Reference(s) ： | 14627 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension |
| Medium ： | 80-90% mixture of MEM with Earles salts + Dulbeccos MEM (at 1:1) + 10-20% h.i. FBS + 2x non-essential amino acids + 2x vitamins |
| Subculture ： | maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:4 every 3-5 days; seed out at 0.5-0.7 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 25-50 hours |
| Harvest ： | saturation density at ca. 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypodiploid karyotype with 20% polyploidy - 30(29-30)<2n>XY, 6-8 centric fusion markers present |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |