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| 产品名称： | RLC-18 |
|---|---|
| 商品货号： | TS259416 |
| <strong>Cell line </strong>： | RLC-18 |
| DSMZ no. ： | TS259416 |
| Species ： | confirmed as rat with IEF of AST, MDH, NP and with species PCR |
| Cell type ： | embryonal liver |
| Origin ： | established from the normal liver of a 17-day rat embryo (strain JAR-2) in 1975 |
| Reference(s) ： | 15003 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like growth in monolayers |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:3 to 1:4 every 3 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm2 + 7-10 ml medium |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-40 hours |
| Harvest ： | harvest of ca. 20 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics ： | rat hypertriploid karyotype with 13% polyploidy - 64(58-66)<3n> - large submetacentric marker present in most cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |