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| 产品名称： | MHEC5-T |
|---|---|
| 商品货号： | TS259421 |
| <strong>Cell line </strong>： | MHEC5-T |
| DSMZ no. ： | TS259421 |
| Species ： | originally confirmed as mouse with IEF of AST, MDH, NP and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | heart endothelial cells |
| Origin ： | spontaneous malignant transformation after homotransplanting MHEC5 NMRI mouse myocardial cells (2-weeks-old), thus forming hemangioendothelioma; cells produce tumors upon injection into histocompatible mice |
| Reference(s) ： | 14854 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells growing as monolayers; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | split confluent culture 1:3 to 1:6 every 3-6 days; use of trypsin/EDTA is not necessary (cells can be detached by tapping the flask or repeated pipetting); however, this results in cell clumps which are difficult to disperse; for single cells the use of trypsin/EDTA is recommended; seed out ca. 0.1 x 105 cells/cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 1 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine flat-moded hexaploid karyotype - 120(96-122)<6n> - 2-3r/dmin and 3 centric fusion markers |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |