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| 产品名称： | MHH-CALL-4 |
|---|---|
| 商品货号： | TS259422 |
| <strong>Cell line </strong>： | MHH-CALL-4 |
| DSMZ no. ： | TS259422 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the peripheral blood of a 10-year-old Caucasian boy with acute lymphoblastic leukemia (pre B-ALL) at diagnosis in 1993 |
| Reference(s) ： | 15018 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, small round cells growing in suspension, partly in clusters; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml (initially in a 24-well-plate is of advantage); optimal split ratio at about 1:2 once a week; culture should not be maintained <0.6 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 80-100 hours |
| Harvest ： | cell harvest of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 +, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 4% polyploidy - 45(42-46)<2n>X, -Y, r(12)(qter;p13-pter) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |