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| 产品名称： | V-79 |
|---|---|
| 商品货号： | TS259423 |
| <strong>Cell line </strong>： | V-79 |
| DSMZ no. ： | TS259423 |
| Species ： | originally confirmed as Chinese hamster with IEF of MDH, NP and as Cricetulus barabensis (Chinese stripped hamster) origin by COI DNA Barcoding |
| Cell type ： | lung fibroblasts |
| Origin ： | established from the lung tissue of a Chinese hamster in 1958 |
| Reference(s) ： | 14504, 14542 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblast-like adherent cells growing in clumps; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 3 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 every 2-3 days; cells can be detached by pipetting with PBS or by mild treatment with trypsin/EDTA; after splitting, it is recommended to seed out the cells in fresh culture flasks as possibly the cells may not attach any longer in the old vessels |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 16-20 hours |
| Harvest ： | cell harvest of ca. 25 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Fingerprint ： | unique DNA profile with (gtg)5 multilocus probe |
| Cytogenetics ： | hamster highly rearranged near-diploid karyotype with 20% polyploidy - 22(20-24) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |