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| 产品名称： | TF-1 |
|---|---|
| 商品货号： | TS259425 |
| <strong>Cell line </strong>： | TF-1 |
| DSMZ no. ： | TS259425 |
| Species ： | confirmed originally as human with IEF of AST, NP |
| Cell type ： | erythroleukemia |
| Origin ： | established from the bone marrow of a 35-year-old man with erythroleukemia (AML M6) in 1987; cell line was first called MFD-1; cells are described in the literature to be proliferatively responsive to several hematopoietic growth factors including EPO, GM-CSF, IFN-gamma, IL-3, IL-4, IL-5, IL-6, IL-13, LIF, NGF, OSM, SCF, and TNF-alpha (see Mire-Sluis et al., J. Immunol. Methods 187: 191-199, 1995); we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14482, 14666, 14665, 14987, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large, single, round cells in suspension; cells shed large amounts of cytoplasmatic particles that accumulate in the culture (not to be confused with bacteria); image; image |
| Medium ： | 60-70% RPMI 1640 + 20% h.i. FBS + 10-20% vol conditioned medium of cell line 5637 (DSM ACC 35) (or 1-5 ng/ml recombinant GM-CSF or IL-3) |
| Subculture ： | seed out at ca. 1 x 106 cells/ml in a 24-well-plate or small culture flask; split saturated culture 1:2 every 3 days; optimal density at ca. 0.5-1.0 x 106 cells/ml. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | maximal density at ca. 1.3-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD71 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged hyperdiploid karyotype with 12% polyploidy - 52-57<2n>XY/XXY, +3, +5, +6, -8, +12, +15, +19, +19, +20, +20, +3mar, der(1)?dup(1)(p21p31)t(1;8)(p36;q11), t(2;12)(q32;q14), t(3;12)(p13-14;p12-13), add(3)(q21), add(5)(q11-13), der(8)t(1;8)(p36;q11), der(12)t(3;12)(p13-14;p12-13)t(1;12)q31-32;q24), add(14)(p12), iso(17)(q10)add(17)(q21), add(19)(q13), trp(19)(q12;q13.3), der(21)t(19;21)(q13.1;q22)dup(19)(q13.1q13.3)t(11;19)(q13;q13.3), der(22)t(19;22)(q11;p11) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |