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| 产品名称： | ST-2 |
|---|---|
| 商品货号： | TS259426 |
| <strong>Cell line </strong>： | ST-2 |
| DSMZ no. ： | TS259426 |
| Species ： | originally confirmed as mouse with IEF of AST, MDH, NP and recently as mus musculus by COI DNA barcoding |
| Cell type ： | stromal cells |
| Origin ： | established from Whitlock-Witte type long-term bone marrow culture of BC8 mice |
| Reference(s) ： | 14811 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, fibroblast-like cells growing in monolayers |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split nearly confluent culture 1:4 to 1:5 every 3-5 days using trypsin/EDTA (cell layer might detach spontaneously when cells grow too densely); seed out 0.5-1.0 x 106 cells/80 cm2 in 7-10 ml medium |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-50 hours |
| Harvest ： | cell harvest of ca. 5 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine flat-moded hypotetraploid karyotype - 68-77<4n> - centric fusion marker present in most cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |