微信小李
微信小章
产品简介
购买须知
| 产品名称： | SGE-1 |
|---|---|
| 商品货号： | TS259428 |
| <strong>Cell line </strong>： | SGE-1 |
| DSMZ no. ： | TS259428 |
| Species ： | confirmed as rat with IEF of AST, MDH, NP, PEP B |
| Cell type ： | kidney glomerular epithelial |
| Origin ： | established from isolated renal glomeruli of a 3-week-old normal male Wistar rat in 1988 |
| Reference(s) ： | 15068, 15069 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing as monolayer with polygonal shapes |
| Medium ： | 45% Dulbeccos MEM + 45% Hams F12 + 10% h.i. FBS + 1x insulin-transferrin-sodium selenite + 10 ng/ml EGF |
| Subculture ： | split culture prior to confluency, 1:10 to 1:20 twice a week using trypsin/EDTA; seed out at ca. 2-2.5 x 104 cells/cm2; cells grow also without coating of the flask with collagen |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 33 hours |
| Harvest ： | cell harvest of ca. 20 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics ： | rat flat-moded hyperdiploid karyotype - 43-48<2n> |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |