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| 产品名称： | M3E3/C3 |
|---|---|
| 商品货号： | TS259433 |
| <strong>Cell line </strong>： | M3E3/C3 |
| DSMZ no. ： | TS259433 |
| Species ： | originally confirmed as Syrian (Golden) Hamster by species PCR and recently as Mesocricetus auratus origin by COI DNA barcoding |
| Cell type ： | fetal lung |
| Origin ： | established from the lung of a Syrian golden hamster fetus on day 15 after gestation in 1981; primary culture cells were treated with hydroxy-proline; cells were described as premature epithelial cells with the ability to differentiate into mucus-producing pulmonary cells |
| Reference(s) ： | 14511 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing in monolayers |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS + 1 mM sodium pyruvate |
| Subculture ： | split confluent culture 1:3 to 1:6 every 3-4 days using trypsin/EDTA; seed out at 1-2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 8 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics ： | near-diploid Syrian hamster karyotype with 10% polyploidy - 43-47<2n> |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |