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| 产品名称： | MHH-CALL-2 |
|---|---|
| 商品货号： | TS259434 |
| <strong>Cell line </strong>： | MHH-CALL-2 |
| DSMZ no. ： | TS259434 |
| Species ： | confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the peripheral blood of a 15-year-old Caucasian girl with acute lymphoblastic leukemia (cALL) in 1993 (at diagnosis) |
| Reference(s) ： | 15018, 16565 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, relatively small round cells growing in suspension; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | cells are difficult to culture; initially after thawing viability may decrease dramatically, but cells recover after 1-2 weeks when seeded out in 24-well plates and at high density: 1-2 x 106 cells/ml; cells grow slowly, split saturated culture about 1:2 every 3-5 days after recovery: do not dilute below 1 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 100 hours |
| Harvest ： | maximal density at ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 +, CD19 +, CD34 -, CD37 -, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cy lambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 13% polyploidy - 52(50-52)<2n>XX, +8, +10, +18, +18, +21, +21 - hyperdiploidy with tetrasomy 21 associated with pre B-ALL in children - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |