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| 产品名称： | MHH-CALL-3 |
|---|---|
| 商品货号： | TS259436 |
| <strong>Cell line </strong>： | MHH-CALL-3 |
| DSMZ no. ： | TS259436 |
| Species ： | confirmed as human with IEF of MDH, NP, PEP B |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the bone marrow of an 11-year-old girl with pre B cell acute lymphoblastic leukemia (ALL) in 1993 (at diagnosis) |
| Reference(s) ： | 15018 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, relatively small round cells growing in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | cells may be difficult to culture; seed out at ca. 1 x 106 cells/ml; cells grow best in 24-well-plates; during the first 2-6 days proliferation rate may be rather low but the medium rarely becomes acidic (yellow), however it is advisable to change the spent medium every 2-3 days; split saturated culture 1:2; minimum density at 0.8 x 106 cells/ml, maximum density at ca. 5 x 106 cells/ml but optimal density at ca. 1-2 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 120 hours |
| Harvest ： | cell harvest of ca. 2 x 106 cells/ml in a 24-well-plate |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, cyCD79a +, CD80 -, CD138 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human pseudodiploid karyotype - 46(45-46)<2n>XX, del(6)(q15), der(9)t(9;9)(p21;q11), der(19)t(1;19)(q23;p13) - carries t(1;19) primary and 6q- secondary rearrangements associated with pre B-ALL - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |