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| 产品名称： | DEL |
|---|---|
| 商品货号： | TS259437 |
| <strong>Cell line </strong>： | DEL |
| DSMZ no. ： | TS259437 |
| Species ： | confirmed originally as human with IEF of AST, LDH, NP |
| Cell type ： | malignant histiocytosis |
| Origin ： | established from the pleural effusion of a 12-year-old boy with malignant histiocytosis at diagnosis in 1987 (problably anaplastic large cell lymphoma, ALCL); initially established by serial passage in nude mice; cells carry the NPM1-ALK (NPM-ALK) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14485, 14492, 14464, 14370, 14737, 15185, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | grows often in clusters in suspension, most cells are polygonal (some cells are round); image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.4 x 106 cells/ml; split saturated culture 1:3 to 1:5 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | maximal density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD5 -, CD6 -, CD7 -, CD8 -, CD10 -, CD14 -, CD19 -, CD25 +, CD30 +, CD34 -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human biclonal, hyperdiploid/hypertriploid, karyotype with 6.7% polyploidy - 47(44-49)<2n>XY, +5, +5, +6, -8, +mar, der(5)t(5;6)(q35;p21), t(5;6)(q35;p21), add(10)(q23-24), der(13)t(1;13)(q32;p11)t(1;13)(q21;q34), add(16)(q23), add(19)(p13), 33% - 74(74-77)<3n>XXY, +1, +3, +5, +5, +6, +7, -10, +13, -18, +20, t(5;6)(q35;p21)x2, add(10)(q24)x1-2, der(13)t(1;13)(q32;p11)t(1;13)(q21;q34)x2, add(16)(q23), add(19)(p13), 60% - matches published karyotype, t(2;5) not detectible cytogenetically |
| Molec. Genetics ： | expression of NPM1-ALK (NPM-ALK) fusion gene was detected by RT-PCR analysis |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV - |