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| 产品名称： | JK-1 |
|---|---|
| 商品货号： | TS259438 |
| <strong>Cell line </strong>： | JK-1 |
| DSMZ no. ： | TS259438 |
| Species ： | originally confirmed as human with IEF of AST, LDH, MDH and recently by STR fingerprinting |
| Cell type ： | chronic myeloid leukemia in blast crisis |
| Origin ： | established from the biopsy material of the shoulder tumor of a 62-year-old man with chronic myeloid leukemia (CML) in erythroid blast crisis in 1987; described in the literature to produce hemoglobin; cells are described in the literature to carry t(9;22) leading to BCR-ABL1 e13-a2 (b2-a2) fusion gene |
| Reference(s) ： | 14824, 14479, 14488 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round single cells growing in suspension (cell pellet shows reddish color hemoglobin) |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml in 24-well-plates; maintain at 0.3-1.0 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days; it is recommended to culture the cells in 24-well-plates continuously |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximal density of ca. 1.2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD71 +, CD235a +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype without significant polyploidy - 48(45-48)<2n>XY, +8, +22, t(1;7)(q10;q10), der(9)t(9;22)(q34;q11), der(22)t(9;22)(q34;q11)x2 - carries two Ph - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |