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| 产品名称： | KYSE-140 |
|---|---|
| 商品货号： | TS259440 |
| <strong>Cell line </strong>： | KYSE-140 |
| DSMZ no. ： | TS259440 |
| Species ： | originally confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | esophageal squamous cell carcinoma |
| Origin ： | derived from the moderately differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 54-year-old Japanese man prior to treatment (depth of invasion was a1); described in the literature to carry p53 mutation |
| Reference(s) ： | 15006, 14955, 14646, 14954 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing as monolayer with the tendency to pile up |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 every 2-4 days using trypsin/EDTA (cells may need five or more minutes to detach from the flask; prior to using trypsin, cells should be washed twice with PBS) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of ca. 20-30 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid/hypotriploid karyotype with 19% polyploidy - 59(56-64)<2n>Xder(X), -Y, add(X)(p1?), del(1)(q32), add(1)(q43), i(3q), add(2)(p2?), del(3)(q22), add(4)(p11), del(4)(q11), add(5)(q1?)x1-2, ins(6;?)(q1?), add(7)(q35)x2, add(8)(q24), del(9)(p11), add(9)(q34), add(11)(p15), add(12)(q24), add(13)(p11), i(13q), add(18)(p12), add(19)(p13), inc. - many additional structural and numerical rearrangements, including multiple insertions, producing an overwhelming preponderance of marker chromosomes |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |