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| 产品名称： | HD-MY-Z |
|---|---|
| 商品货号： | TS259444 |
| <strong>Cell line </strong>： | HD-MY-Z |
| DSMZ no. ： | TS259444 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | Hodgkin lymphoma |
| Origin ： | established in 1991 from the pleural effusion (rich in Hodgkin/Reed-Sternberg cells) from a 29-year-old patient with nodular sclerosing Hodgkin lymphoma (stage IIIb) refractory to therapy |
| Reference(s) ： | 14371, 14478 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | variable morphology; semi-adherent, spheroid to fusiform cells growing in clumps in mono- and multilayers, occasional giant cells present; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | maintain at 0.2-0.75 x 106 cells/ml or 0.8-1.6 x 105 cells/cm2; optimal split ratio of 1:3 to 1:8 every 2-5 days; cells may be dislodged mechanically without trypsin/EDTA; seed out at ca. 0.3-0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | saturation density at ca. 1.5 x 106 cells/ml or 5 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 -, CD3 -, CD10 +, CD13 +, CD14 -, CD15 +, CD19 -, CD21 -, CD25 -, CD30 -, cyCD68 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged hyperdiploid karyotype with 24% polyploidy - 52-55<2n>X/Xdel(X)(p11), +1, +2, +5, +6, +8, +9, -17, -17, -18, +19, +20, +21, +22, +2mar, der(1)t(1;3)(q32;p21), der(2)t(2;?10)(p25;q22), add(3)(q27), del(4)(q22), i(5p), i(5q), der(5)t(5;8)(p14.1;p12), del(6)(q15), add(7)(p15), add(7)(p11), del(8)(q11), der(9)add(9)(p24)add(9)(q21), der(10)add(10)(p11)add(10)(q22), add(11)(q24), i(13q), der(13)t(7;13)(q11;p13), add(18)(p13), add(19)(q13.1) - additional rearrangements present in subclones include der(1)t(1;2)(q11;p25), del(3)(q21), add(16)(q24) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |