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| 产品名称： | MH-7777A |
|---|---|
| 商品货号： | TS259445 |
| <strong>Cell line </strong>： | MH-7777A |
| DSMZ no. ： | TS259445 |
| Species ： | confirmed as rat with IEF and by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | hepatoma |
| Origin ： | established from the liver tumor of a Buffalo rat; tumor cells were propagated in the hind leg |
| Reference(s) ： | 14544, 15044 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, polymorph round cells growing in monolayers |
| Medium ： | 90% Dulbeccos MEM + 10% horse serum + 4 mM L-glutamine + 10 nM insulin + 10 µM dexamethasone |
| Subculture ： | split nearly confluent culture 1:4 every 3-4 days using trypsin/EDTA, cells should not be grown to full confluency as they tend to detach; seed out at ca. 3 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of ca. 1.5 x 107/80 cm2 |
| Storage ： | frozen with 70% medium, 20% horse serum, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | near-tetraploid rat karyotype with 17% polyploiy - 73-82<4n> |
| Viruses ： | ELISA: reverse transcriptase negative |